Antigenic markers on fragment DD, a unique plasmic derivative of human crosslinked fibrin
نویسندگان
چکیده
منابع مشابه
Antigenic markers on fragment DD, a unique plasmic derivative of human crosslinked fibrin.
Plasmic degradation of crosslinked human fibrin produces unique degradation products. (DD)E complex and fragment DD, in which the DD moiety contains two fragment D molecules joined together by covalent bonds. In this study. fragment DD antigenic markers were studied using two different antisera: one against intact (DD)E complex, and the other against fragment DD that has been exposed to 3 M ure...
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After addition of a low concentration of thrombin to normal plasma, a progressive and significant increase in crosslinked fibrin polymers was found by sodium dodecyl sulfate agarose gel electrophoresis, reaching 27% of total fibrinogen and fibrin before gel formation. As measured by enzyme-linked immunosorbent assay with a monoclonal antibody specific for an epitope near the yy crosslink site, ...
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Proteolysis of human cross-linked fibrin by plasmin results in the formation of a DD . E complex, and Fragments DD and E as the major degradation products. Three species of Fragment E, which differ both in molecular weights (E1, Mr = 60,000; E2, Mr = 55,000; E3, Mr = 50,000) and in charge, have been isolated from a digest of cross-linked fibrin. Each Fragment E species reacts with monospecific ...
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We have previously demonstrated that increasing factor XIII concentrations above that present in plasma (1 U/mI) results in the formation of very high molecular weight a fate polyacrylamide and agarose gel electrophoresis (SDSPAGE). In this report. we have examined the effect of such crosslinking on plasmic susceptibility of fibrin prepared from purified fibrinogen and from plasma in the presen...
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ژورنال
عنوان ژورنال: Blood
سال: 1979
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v54.4.794.bloodjournal544794